Hélène Perreten Lambert
Marsens
Profil professionnel
Passionate PhD biologist with strong interest for lab technical work, with more than 25 years of experience in research laboratories. Specialized in molecular biology, cell culture, viral production and biochemistry techniques with a strong commitment to accuracy, reproducibility and laboratory safety standards. Seeking to continue developing advanced laboratory skills while contributing to impactful research projects.
Vue d'ensemble
3
3
Languages
11
11
years of post-secondary education
22
22
years of professional experience
Expérience
laboratory technician
EPFL
Lausanne
2011.04 - Current
- cloning: mutagenesis, GIbson Assembly, siRNA, CRISPR
- DNA, RNA and protein extraction
- culture of various mammalian cells, transfection, generation of stable cell lines with lentiviral systems
- western blot
- immunofluorescence and light microscopy imaging
- management of ordering laboratory materials and equipment
- Biosafety Officer and CoSeC
PhD Student
EPFL
Lausanne
2005.01 - 2010.09
- studies on astrocytic mitochondrial uncoupling proteins on neuronal survival.
- use of lentiviral systems
- primary cultures of astrocytes and neurons from newborn and fetal mice brains, astrocytes/neurons co-culture
- biochemical assay: lactate release, labeled glucose uptake, glycogen content, protein content, cell survival, oxygen consumption, mitochondria membrane potential
- teaching assistant
Formation
Doctorate - Neuroscience
EPFL
Lausanne 2005.05 - 2011.01
Master's degree - Biology
Centre médical universitaire
Genève 2003.01 - 2004.10
Bachelor's degree - Biology
Université de Genève
Genève 1999.09 - 2002.10
Compétences
- cell culture: cell lines and primary cells
- molecular biology and biochemistry techniques
- Ability to work both independently and as a part of a team
- organization skills: time management, multi tasking
- Detail-oriented
- curiosity
- compute skills: Microsoft Office, Snapgene
Language
French
NATIVE LANGUAGE
English
Working proficiency
German
Basic knowledge
Chronologie
laboratory technician
EPFL
2011.04 - Current
Doctorate - Neuroscience
EPFL
2005.05 - 2011.01
PhD Student
EPFL
2005.01 - 2010.09
Master's degree - Biology
Centre médical universitaire
2003.01 - 2004.10
Bachelor's degree - Biology
Université de Genève
1999.09 - 2002.10